Exact(36)
Relative induction level was relative to the corresponding non infected sample at each time point analyzed.
RuACS accumulation is reduced in the Bowman- Rph15 NIL infected sample (protein spots numbers 2577, 2581).
Few studies have investigated the relationship between maternal PTSD and child behaviour outcomes in an HIV vertically infected sample.
The Ct (cycle at which the increase of fluorescence exceeded the threshold setting) was used to calculate the fold changes (FC) in each infected sample compared to the expression level detected in the corresponding sample under control conditions (baseline) with the following formula: FC = 2 -ΔΔCt where ΔΔCt = (Ct target - Ct actin) infected sample - (Ct target - Ct actin) uninfected sample.
Dye-swap hybridization was performed, i.e. S. aureus infected sample was labeled with Cy5 and the respective uninfected control sample was labeled with Cy3 and hybridized together to one array.
We extracted hemolymph from approximately 200 D. virilis females 24 hours after infection with a mixed culture of E. faecalis and S. marcescens (infected sample) and 200 uninfected controls (uninfected sample).
Similar(24)
When calculating relative changes to the pre-infection samples, infected samples were not subtracted from their individual uninfected controls (since not every infected sample had a pre-infected control), but from an average of all the pre-infection samples.
Although the array data for these genes suggested a negligible and virtually identical presence of blood in both control and infected samples, RT-qPCR analysis revealed a small blood contamination (CT ≥ 34 for ALAS2, CT ≥ 36 for RHAG) within two samples, one control (CovD1) and one infected sample (A4) (Additional file 10 and Additional file 11).
'Pooled' sample contains lysates from independently grown FLAG -and HA-tagged viruses, whereas 'co-infected' sample was obtained from cells infected with both FLAG -and HA-tagged variants.
As shown in Figure 1, there was slight increase in the unigene expression in the NNV-infected sample at 6 hr post-infection, as indicated by the RPKM values scattering toward the X-axis (NNV-infected sample).
To rule out false negatives in SCSMV infection, in addition to using SCSMV-uninfected sugarcane sample as negative control, SCSMV-infected sample and water were also used as positive and blank controls.
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