These patients are infected by replication competent virus strains.
Sub-confluent C2C12 myoblasts are infected with replication defective recombinant adenovirus (Ad-Unc45bFlag/GFP) at 5×108 pfu/ml in growth medium (90% DMEM, 10% FBS) by incubation of 2 ml of virus per p100 dish of cells for 2 hr at 37°.
Most such patients are nonetheless infected by replication-competent viruses.
For transient gene expression, HUVECs were infected with replication-deficient, recombinant, Adenovirus vectors carrying cDNAs expressing human sense (Ad.sp21) p21[21] and GFP RNA (Ad.GFP) as previously described[3].
This issue was resolved in part by the identification of a small, stable pool of resting CD4+ T cells latently infected by replication-competent HIV [1] [3].
Plated cells were infected with replication-competent HIV-1TT31, HIV-1JRFL, or HIV-1IIIB at multiplicities of infections of 0.004, 0.02, or 0.05 respectively.
Several studies have demonstrated that some individuals have been infected by replication-incompetent viruses, which have unusual polymorphisms in nef [5], [6], vpr [7], and other genes [8].
YES-2 cells were infected with replication-competent AdF35 and with Ad5/p53 or Ad5/LacZ, and then cell numbers were counted.
Nevertheless, when MSCs are infected with replication-competent Ad, cell-mediated immunity against viral gene-loaded MSCs will be generated.
Briefly, passage 1 HUVECs were seeded on cross-linked gelatin at a cell density of about 3×10 and subsequently infected with replication-deficient lentivirus carrying Lifeact-EGFP.
Ad E1A expression was only detected in the groups infected with replication-competent Adwt, dl1520, and Ad-cycE, but not in the groups mock-infected or infected with AdGFP (Fig. 4b).
