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The mPCR assay was validated using naturally infected field samples for one or more citrus viruses and the huanglongbing bacterium.
While studies of the disease in naturally infected field cases of bovine tuberculosis have provided valuable information, detailed knowledge can also be gained through studies of disease models.
The reliability of the standard RT-PCR and nPCR was demonstrated using a range of cultivars collected from an infected field site.
These figures are in good agreement with sequencing data of 60 PLMVd-sRNAs from infected field trees [26], although there are major differences in the methodologies and inferences between both studies (see below).
The partial msp4 sequences obtained from 48 infected field voles were all indistinguishable from one another.
Stunting is a common symptom in greenhouse or growth chamber experiments with V. longisporum occurring already at the rosette stage, but is never seen in infected field crops.
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It costs $8 to $10 an acre to treat infected fields, he said, and for an average-sized farm near this community of 3,000, that can quickly add up to $6,000 to $10,000 a year.
Though it has not yet appeared in Latin America, the biggest banana-producing continent, that seems only a matter of time.At the moment, the only way to cope with the disease is to quarantine infected fields when the problem first appears and to ensure that all seedings of new plants are clean.
Maize plant samples, showing typical late wilt symptoms, were collected from naturally infected fields located at Gharbia Governorate, Egypt.
Infection at maximum tillering stage results in blighting of leaves, which eventually causes significant yield losses in severely infected fields ranging from 20 to 30%, but this can reach as high as 80% (Mew et al.1992; Noh et al.2007; Shin et al.1992).1992
This disease can provoke yield losses of up to 40% in highly infected fields.
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