Sentence examples for infected by adding from inspiring English sources

The S2R+ cells were then infected by adding 10 µl of a suspension of GFP-expressing F. tularensis ssp.

Cells were infected by adding 20 µl of the pre-incubated treatment to the 280 µl of media, thus diluting the treatment and/or virus 15-fold.

Subsequently, cells were infected by adding FMDV A24 Cruzeiro (MOI of 0.01) and incubated for 1 hour at 37°C to allow for virus adsorption.

The skin was infected by adding 1×106 CFU of an overnight culture of S. aureus ATCC 29213 and E. coli ATCC 25922 in a total volume of 10 µl.

The wounded area was infected by adding 1×106 cfu of an overnight culture of P. aeruginosa 15159 (clinical chronic ulcer isolate) or S. aureus ATCC 29213 in a total volume of 100 µl of human serum diluted in phosphate buffered saline (50∶50).

MSCs were infected by adding viral supernatants to the culture medium.

Health workers, too, have been affected and infected by HIV adding further stress to the provision of health services.

Free parasites were prepared from mature infected erythrocytes by adding 10 volumes 0.02% (w v-1) saponin in cold PBS (116 mM NaCl, 8.3 mM Na2HPO4, 3.2 mM KH2PO4, pH 7.4) for 1 min, followed by two washes by centrifugation for 5 min at 4°C at 1260× g and resuspension in PBS.

"One in 20 young people aged 20 to 29 years old is infected by HIV," added Mr. Abai.

The number of individuals known to be HIV infected was calculated by adding the number of newly diagnosed cases during that year to the number of individuals known to be HIV infected during the previous years and subtracting the number of people who died or left Israel.

Cells were plated in DMEM supplemented with 10% FBS and 1% Penicillin-Streptomycin on day -1 and infected with lentiviruses in the presence of 8 μg/ml Polybrene (Millipore, Billerica, MA) on day 0. Infected cells were selected by adding Puromycin (Santa Cruz, Biotechnology, Dallas, TX) to a final concentration of 2 μg/ml on day +1.