Generally, such artificial receptor proteins should be based on monomeric and small polypeptides that are robust, easily engineered, and efficiently produced in inexpensive prokaryotic expression systems.
The robustness of the Top7 scaffold under extreme conditions of pH and temperature and its amenability to production in inexpensive bacterial expression systems reveal great potential for novel biotechnological applications.
"After speaking with several friends and associates in Tokyo, I knew that the ideal gift would be the relatively inexpensive, aesthetic expression of caring and hope," said Strong, owner of Memoirs.
This PCR strategy allows a rapid and inexpensive approach for intracellular expression of si⧸shRNAs and subsequent testing of target site sensitivity to downregulation by RNA interference (RNAi).
This method allows rapid and inexpensive comparison of gene expression across a large number of samples.
Additionally, the CRISPR Cas9 system is relatively inexpensive as the Cas9 expression vector is reused and only new sgRNA constructs need to be cloned for every target sequence at the cost of a few oligonucleotides.
A label called Slow and Steady Wins the Race recently produced a series of $100 handbags that recreated the shapes of iconic designs using inexpensive canvas — "a visual hyperbolic expression about contemporary fashion's attention and obsession with designer handbags," says its Web site.
This technique is a relatively inexpensive technology for assaying the expression of a small number of genes.
Our method is very simple and inexpensive, since only requires transient expression of the test proteins and a regular fluorescence microscope.
Promising new research suggests that immunohistochemistry (IHC) for MLH1 and MSH2 expression is an inexpensive first screen of CRC tumors to evaluate whether MSI and/or germline testing is indicated.
Plants have been studied extensively for the production of pharmaceutical proteins, as they represent an inexpensive and scalable alternative to common expression systems.
