Sentence examples for inefficient expression from inspiring English sources

To insure that the X-ray sensitivities of the nuclease-dead mre11 mutants were not the result of inefficient expression due to employing extrachromosomal plasmids to express the mre11 alleles, the mre11 nuclease defective genes were integrated into the chromosome by two-step gene replacement.

However, these treatments can result in non-homogenous and/or inefficient expression of therapeutic proteins in vivo due to impeded diffusion and dilution of the inducer further from the injection site.

Inefficient expression could explain the reduced retrotransposition activity observed for the ORF2 chimeric protein.

Therefore, we reasoned that the inefficient labeling of Z/EG and Z/AP granulocytes may be due to impaired recombination and/or inefficient expression of the transgenic loci.

The relative intensities of the two bands indicated that deletion of Ndst1 in Ndst1f/f MMTVCre+ was as high as ∼80% in multiparous females (Fig. 1C), suggesting that some cells escaped recombination, possibly due to inefficient expression of Cre.

A similar correlation between hypermethylation of the pCAGGS promoter and inefficient expression of the Cre-reporter gene was also observed in granulocytes obtained from LysM-Cre/Z/AP mice (Figure 4C, 4E, and 4G).

However, they are often inefficient for expression of soluble antibody fragments, and sub-cloning of selected antibody populations into dedicated soluble antibody fragment expression vectors can enhance expression.

Similarly, gene organization of highly expressed genes is expected to be under stronger purifying selection because the cost of inefficient tuning of expression levels in highly expressed proteins is presumably higher.

The mammalian type I gonadotropin releasing hormone receptor (GnRH-R) is a structurally unique G protein-coupled receptor (GPCR) that lacks cytoplasmic tail sequences and displays inefficient plasma membrane expression (PME).

However, certain limitations apply to most of these systems, including leakiness of transgene expression, inefficient transgene silencing or activation, as well as limited tissue accessibility of transgene-inducers or their unfavourable pharmacokinetics.

A systematic analysis of Lhx2 function in ES cells differentiated in vitro using retroviral vectors is not possible due to the inefficient and unpredictable expression pattern from such vectors in the ES/EB system [41].