Inefficient photoreceptor differentiation was reported in studies that exposed cultures to retinal differentiation conditions for 2 3 weeks (Cicero et al., 2009; Gualdoni et al., 2010).
Developments in the generation of iPSCs have advanced significantly in the past 6 years ; however, concerns related to potential tumorigenicity, incomplete and inefficient terminal differentiation, as well as immunogenicity of iPSCs and in particular their differentiated progeny remains to be fully elucidated.
Inefficient neural differentiation of human induced pluripotent stem cells (hiPSCs) motivates recent investigation of the influence of biophysical characteristics of cellular microenvironment, in particular nanotopography, on hiPSC fate decision.
Interestingly, we found that addition of Wnt3a can rescue cultures of H7 human ES cells that demonstrated inefficient cardiac differentiation in response to activin A/BMP4 (Figure 2B).
Furthermore, addition of exogenous Wnt3a was able to rescue inefficient cardiac directed differentiation in some cases, increasing the percent sMHC+ cells by more than 6-fold (Figure 2).
DPY-30 or RBBP5 knockdown reduces global and neuronal gene-specific H3K4me3 levels, resulting in inefficient RA-induced neural differentiation of mouse ES cells.
At least the viral cDNA synthesis and integration seem to be per se extremely inefficient in monocytes since differentiation-dependent cofactors of reverse transcription might be limited [4], [7].
However, many organoid differentiation protocols are inefficient and display marked variability in their ability to recapitulate the three-dimensional architecture and course of neurogenesis in the developing human brain.
Human pluripotent stem cell (hPSC -derived endothPSC -derivedendothelialstitutes a promising source for therapeutic revasculineageion, but progress in this arena has been hampered by a lacells constitutesscalable differentiation promising and inefficient formation of a functional vesourceetwork integrating with the host circulation upon transplantation.
Unfortunately, iPS cell cardiomyocyte differentiation ratio is extremely inefficient for therapies, much like ES cells [26].
