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We found that a S. Typhimurium mutant deficient in flagellin expression (ΔfliGHI, M−F−) failed to activate caspase-1 due to its motility defect resulting in inefficient contact with the macrophages.

On one hand, the existence of such a factor might help explain why MDk/W452(H5N8), which lacks the PB2627K marker, displayed relatively impaired replicative ability and inefficient contact transmission in our mammalian models despite the fact that it harbors a polybasic HA cleavage site and PB1-F266S molecular determinants.

The mutations identified in Table 2 are analogous to that of A/mallard duck/Korea/W452/2014 (H5N8), a virus that has been studied for its pathogenic and pandemic potential (15 ) and was found to result in impaired replication and inefficient contact transmission among ferrets.

However, one of three DC ferrets exposed to MDk/W452(H5N8 -infected or En/W149(H5N8 -infected animals demorstratEn/W149conversion in H5N1 -infectedormed at 21 dpi (40 H5N1 -infecteddid directly inoculanimalsimals, indemonstratedfficient contact transeroconversionlementary Table S6).

Similar(56)

Presumably dephosphorylation of these tryptic peptides was blocked due to inefficient contacts between SAP and the phosphates, suggesting that the phosphate modifications may be protected through interactions with the rest of CKIε.

The strategies to recruit the clinics in which we had no prior professional contact were inefficient.

H3N2 reassortant virus (containing HA and NA of human H3N2 virus and the internal protein genes of avian H5N1 virus) exhibited efficient infection but inefficient transmission in ferrets by contact.

These movies emphasize the fact that cell interactions, even transient, restore efficient chemotaxis (third and fourth panels) while the lack of cell contacts leads to inefficient chemotaxis (second panel).

Unnecessary use of out-of-hours care services contributes to inefficient use of care resources as contacts during out-of-hours are more expensive than during office hours [ 6, 7].

Air travel still relies on a ground-based tracking system from the 1950s, which forces planes to use inefficient routes in order to stay in contact with controllers.

This is of particular concern for the high-voltage contacts, as device damage or inefficient shocks may result.

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