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A 100 mL-culture of clone FUSAOX was grown under inductive conditions as described previously.
These reports provide evidence that AP2 genes preferentially block the floral transition under inductive conditions.
One clone named FUSAOX (Fig. 3a) was selected and to detect enzyme production it was grown in liquid medium under inductive conditions.
Because cells producing CBHII produce a halo that cannot be distinguished from that produced by the negative control the supernatant from clones transformed with pPIC9- cbhII were grown under inductive conditions and analyzed by SDS-PAGE.
Chondrogenic differentiation within these cell-hydrogel pellets was compared against that of standard cell pellets under inductive conditions and the matrices which supported chondrogenesis were used in the cartilage phase of biphasic constructs.
For osteogenesis and chondrogenesis, inductive conditions were already reported [46].
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After 6 weeks culture of BMMSCs under osteogenic inductive condition, calcium deposits were detected by staining with 1% alizarin red (Sigma-Aldrich Co).
The overexpression of the FPF under inductive condition led to early flowering in tobacco [ 33].
It is also possible that an inductive condition to flower is required for the up-regulation of this gene in wheat.
The stage of SAM development during cold treatment was determined by dissection of a minimum of three shoot apices from the main stem of plants grown at 20°C (floral inductive condition) for 10 or more days after they were removed from vernalization at 6°C.
It is widely perceived that plants do not flower even under photo-inductive conditions until they accumulate enough sugar reserves for the induction of flowering [ 6- 8], which is consistent with the observations that low-starch-containing mutants, such as pgm1 and pgi, exhibit retarded growth and delayed flowering [ 9, 10].
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