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The lack of a decent induction window in case of survival kinetics can be understood in light of product accumulation.
On the basis of these preliminary studies, further differentiation experiments were performed in which the growth factors were included prior to, during and after the hypothesized optimal Mixl1 induction window for each growth factor.
This confirmed that the phenotype in the dox-treated IKMV mice occurred within the 3 day induction window.
It has been shown that the induction window for acute cardioprotection is too short to permit synthesis of new protective proteins [ 26].
Because of the reduced need for sample processing (e.g., no OD600-dependent induction window) and ease by which the culture size can be scaled-up, this system is a very attractive method for achieving high-throughput protein expression.
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The induction windows were reported around 10 100 µM for CdCl2 and arsenate consistent with the EGFP inductions observed between 10 and 100 µM on the Cell-on-Chip device.
With our newly created Tbx4 lung enhancer-driven Tet-On inducible system, lung mesenchymal cells can be specifically and differentially targeted in vivo for the first time by controlling the doxycycline induction time window.
Therefore, with our newly created Tbx4 lung enhancer-driven Tet-On inducible system, lung mesenchymal cells can be specifically and differentially targeted for the first time by controlling the doxycycline induction time window.
In the second sensitivity analysis to evaluate the appropriateness of the induction period window, we moved the index date to 2 years before the liver cancer diagnosis date and reassessed exposure status.
We then looked at lung specimens at different developmental stages in combination with different Dox induction time windows.
Besides the different transgenic strains and induction time windows, the apparent discrepancy is probably due to the relatively short time frame of the previous study, which may have missed the ultimate fate of chondrocytes.
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