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Mitochondrial membrane potential, an early marker of apoptosis induction was assessed using Flowcytometer based Mitolight™ Apoptosis Detection Kit (APT142, Chemicon, USA).
Susceptibility to arrhythmia induction was assessed using both rapid pacing and premature stimuli (S1S2) protocols with the pacing electrodes defined above.
When the frequency of DNA dsb induction was assessed using a mathematical model based on the DNA fragment size distribution into the gel lane, we found a statistically significant relationship between the number of DNA dsb induced and the corresponding alpha-values and fraction surviving after 2Gy (P = 0.0049 and P = 0.0031 respectively).
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To demonstrate MSE or GC-mediated apoptosis induction, apoptosis was assessed using a panel of human cancer cell types, derived from the pancreas (PANC-1), colon (HT-29), breast (MCF-7), and prostate (PC-3), including nonmalignant cells (HEK-29T) treated with or without the IC50 concentrations of MSE or GC (Table 1).
The induction of apoptosis was assessed using the APOPercentage assay (Biocolor Ltd., UK).
The involvement of Seladin-1 protein on induction by goniothalamin was assessed using Western blotting (as shown in Figure 4).
Induction of apoptosis was assessed using Annexin V apoptosis detection kit (BD Biosciences, Heidelberg, Germany) according to the manufacturer's instructions.
At day 7 after osteogenic induction, in vitro mineralization of cells was assessed using the OsteoImage Mineralization Assay kit (Lonza, Basel, Switzerland) according to the manufacturer's manual.
The effect of arthritis induction on body weight, autoantibody production, and cytokine production was assessed using an independent-samples t test (SPSS Inc., Chicago, IL, USA).
Subjective sleepiness was assessed using the Stanford Sleepiness Scale.
Depression was assessed using the MADRS [22].
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