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The formation temperature, maximum induction temperature and induction time are measured.
From the fractional factorial design, the interactive effect of induction temperature and time was the most significant.
Seventeen experiments were designed and conducted for investigation of cell density to start induction, induction temperature, and induction time.
To achieve high levels of soluble form of recombinant human growth hormone (rhGH) we used suitable host strain, appropriate induction temperature, induction time and culture media composition.
The expression levels of MaSp2 with molecular weight of 28.3 256.5 kDa were significantly elevated by down-shifting the induction temperature.
Combination of induction temperature downshift with the glycyl-tRNA pool increase in E. coli led to enhanced biosynthesis of glycine-rich silk proteins.
Similar(6)
The induction conditions included cell density prior induction (OD600nm), post-induction temperature, IPTG concentration and post-induction time.
Accordingly, the optimum Vip3A16 toxin production was 170 μg/ml at 18 h post-induction time and 39 °C post-induction temperature.
We investigated effects of host strain, plasmid, inducer concentration, pre-induction temperature and cell density at induction with design of experiment (DOE).
A post-induction temperature of 25°C was used.
(b) Post-induction temperature and post-induction period at fixed level of inducer concentration.
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