Sentence examples for induction screen from inspiring English sources

We used passive accumulation devices (SPMD and POCIS) to sample contaminants from nine breeding sites of the Common toad (Bufo bufo) across England and Wales, measuring endocrine activity of the extracts in a recombinant yeast androgen screen (YAS) and yeast estrogen screen (YES) and an in vitro vitellogenin induction screen in primary culture of Xenopus laevis hepatocytes.

Another compound validated in our induction screen, previously used in studies with RBC, is sorafenib tosylate (Bay 43 9006).

For the induction screen of the Kinase Inhibitor Library we selected hits for further validation based on z-score, which accounted for 21 compounds in total.

First, we performed four primary screens: LOPAC inhibition and induction screens and Kinase Inhibitors Library inhibition and induction screens.

As inducer of vesiculation, ionomycin was used as a positive control in all induction screens.

We performed inhibition and induction screens in parallel since we aimed at identifying molecules that induce RBC vesiculation (induction screens) and molecules that inhibit vesiculation induced by ionomycin (inhibition screens).

All validated hits from LOPAC and kinase inhibitor induction screens are listed in Tables 1 and 2 respectively.

In general, in case of induction screens, the 30 highest values were selected for validation and in case of inhibition screens the lowest 30.

After analysis of the induction screens, we identified 12 compounds from LOPAC inducing cell shrinkage/vesiculation and 10 compounds from kinase inhibitor set inducing cell shrinkage/vesiculation.

Most compounds were considered validated hits when their median of the replicates (Rep Median score) was smaller than 1, in case of inhibition screens and higher than 1 in case of induction screens and the adjusted P-value (Padj score) was smaller than 0.1 with the exception of eight compounds which were validated according to cell scatter.

The adjusted P-value (Padj score) of all hits in the induction screens was ≤0.1, which was considered significant, with the exception of bromoacetyl alprenolol menthane and NNC 55 0396 from the LOPAC (Table 1) and AT7867 and NVP-TAE684 from the Kinase Inhibitor Library (Table 2).