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This sequence of events was quantitatively monitored (see Fig 4c, Movie S3 and time traces in 5a) by defining appropriate times: t0, beginning of induction pulse; t1, Whi5 nuclear exit; t2, bud emergence, t3, division.
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We then show that periodic 10 20 minutes gene induction pulses can drive many cell division cycles with complete coherence across the cell cluster, with either a G1/S trigger (cln1 cln2 cln3 MET3-CLN2) or a mitotic trigger (cdc20 GALL-CDC20).
For induction, a pulse of methanol was added to the steady-state cultures to produce a final concentration of 1.0 g L−1 and the time of addition defined as time 0. From this time, sorbitol methanol medium was supplied to the fermentor at the same dilution rate used prior to induction, resulting in a smooth transition to the inducible conditions.
To investigate the phenotypic influence of ECM-mediated (produced after TGF-β1 pulses) myofibroblast induction, fibroblasts were pulsed with or without TGF-β1 for 4 h and 2 × 4 h.
In this research, CJ400 electrical contact was used as samples to be welded in the way of pulse induction brazing.
This contribution describes the experiments on pulse induction by cycling the liquid feed in a column of 3.2 m height.
However, in order to show the coexistence of two distinct period distributions for the same dMn values, the DNA repair process triggered by cell irradiation has to be slow (i.e. dMn decreases slowly as DNA damage is repaired) compared to the time required to reach the stationary regime after a p53 pulse induction.
qRT PCR following pulse induction of each Qrr sRNA was used to verify the microarray results.
Upon arrival in the anesthesia induction room, ECG, pulse oximetry and non-invasive blood pressure were monitored continuously.
When we tested two pulse induction in vivo, we ran simultaneous experiments with zero, one, and two 30 min pulses of aTc (50 nM).
MMF and AZA were also compared for maintenance treatment of LN after induction treatment with pulse intravenous CY by Contreras et al 17 Patients included in this trial were almost entirely non-Caucasians and had severe LN.
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