Sentence examples for induction concentrations of from inspiring English sources

The conditions for overexpression of soluble ArtinM were optimized testing different parameters: temperatures (20, 25, 30 or 37°C) and shaking speeds (130, 200 or 220 rpm) during induction, concentrations of the induction agent IPTG (0.01-4 0.01-4d periods of induction (1-19 h).

When conditions similar to those used for the expression and purification of the full-length enzyme did not yield soluble NTD, variations in temperature post induction, concentration of IPTG for induction and total culture volume were implemented.

Then they were transformed to duplicate LB-agar plates containing a low induction concentration of IPTG (0.02 mM) to avoid significant changes of the protein expression.

Suzuki et al. (2013) also observed a dose response relationship between PPARγ2 and BDE-47 using a 5% induction concentration of 10 μM in a human osteosarcoma (U2OS) cell-based reporter assay, but they observed no activity for other BDEs.

In our study, different conditions were assayed to determine those able to provide optimal overexpression of soluble ArtinM and four parameters were tested: temperature and shaking speed during induction, concentration of the induction agent (IPTG) and period of induction (for details see Methods).

In order to evaluate the effect of growth hormones on callus induction different concentrations of 2,4-D alone was incorporated in different media – MS, B5 (Gamborg et al. 1968) and N6 (Chu 1978).

In order to increase the level of induction, various concentrations of methanol (from 0.5 to 3%%) were also tested.

Similar to the observation under SNP induction, all concentrations of PBE and two concentrations of catechin (1 and 5  μg/mL) inhibited the Ca2+ increase in DCFH oxidation to DCF.

Because in our system induction and metabolic properties are separated, we adjusted the model to include IPTG induction and anti-induction for high concentrations of Pgal (Additional file 1), using independent measurements of the expression levels of LacZ.

Two ml of this bacterial preculture were transferred in 50 ml LB medium and grown for 3 h at 28°C prior to an isopropyl-β-D-thiogalactopyranoside (IPTG) induction (final concentration of 1 mM) during 8 h at 28°C.

Panel A shows the average mammary tumor onset as measured in days post IGF-IR transgene induction using concentration of doxycycline ranging from 100 mg/kg to 2000 mg/kg.