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After inducing with IPTG, the expression product was efficiently secreted into the culture medium and shake-flask culturing gave a yield of approximately 5×105 ATU/L (∼60 mg/L).
The duet was described as "scorching" and "chill inducing" with many critics considering the performance to be a show highlight; Lily Harrison of E! added that the pair "set the bar impossibly high".
Proteins were expressed by growing BL21 transformants to approximately OD600 0.6 and inducing with IPTG to 1 mM.
The Sak-Myc protein was overexpressed from pKTH3834, a derivative of pEPSA5 carrying the sak-myc gene under the control of PT5X, by inducing with 0.5% xylose.
By inducing with IPTG, SUMO-FGF21 was expressed at a high level.
In general, there are a variety of binding sites and for every one of them a TF will have a different affinity, inducing, with some probability, transcription.
Similar(45)
It is clear that antibody-induced arthritis induced with antibodies to CII shares many characteristics with that induced with G6PI.
The animation is, as ever, gasp-inducing with dazzling effects of light and detail that we have almost, but not quite, got blasé about.
The few bouts I witnessed in the schoolyard were bloody, fear-inducing, with the sound of fists hitting flesh and bone and — like a scene from "The Sopranos" itself — impossible not to watch.
She could have active labor induced with Pitocin.
Hypertension was induced with norepinephrine.
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