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One of the main ones is "Isolation: inducing loss of reality by physical separation from family, friends, society and rational references".
An alternative technique has been used for phenotypic screening directly from somatic cells -- inducing loss of heterozygosity of the mutated gene, which is a less demanding technique with the application of high-concentration G418 selection in heterozygous cells (Huang et al. 2012).
We propose that sustained Rac activation in CD3-activated cells prevents migratory polarity by inducing the formation of multiple protrusions, increasing microtubule stability and inducing loss of the uropod via decreased ERM phosphorylation.
The up-regulation of miR-146a in TWEAK-treated C2C12 suggests that one of the potential mechanisms by which TWEAK might be inducing loss of skeletal muscle mass is through down-regulation of Numb and TRAF6.
To induce behavioral changes similar to the dyskinesias observed in PD patients [22], [23], [24], [25], [26], we employed the well-established neurotoxic rat model of PD, in which unilateral infusion of 6-OHDA into the medial forebrain bundle, inducing loss of dopamine-containing neurons in the nigrostriatal system, was followed by chronic L-DOPA treatment.
Decreased expression of gastrokine-1 and the trefoil factor may thus favour the development of gastric cancer by weakening the actin assembly (stress fibers) and disrupting smooth cell-substratum contacts, or inducing loss of anchoring and thus anoikis, a form of apoptosis [29].
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Distribution and orientation of gene trap virus insertions were, furthermore, consistent with a high likelihood of inducing loss-of-function mutations.
We also reverted the phenotype of heterozygous luc tXA9 mutants to wild-type by inducing loss-of-function mutations with the CRISPR/Cas system on the chromosome carrying the dominant allele.
AKT1 ablation induced loss of cell adhesion and subsequent apoptosis.
Transient and near-complete loss of PTEN expression induces loss of adhesion of the cells.
Treatment with nutlin-3, an E3 ligase inhibitor, prevented Tat-induced loss of presynaptic terminals.
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