In combination with the appropriate co-stimulatory signals, IL-21 is capable of inducing class-switching and B cell proliferation independent of antigen binding and B cell receptor (BCR) signaling [ 9].
In this report, the use of DNA vaccination to induce class II restricted antigen specific proliferative responses was studied.
Furthermore, the data do not address how the IL-10-producing FOXP3-positive cells might influence the IgE-dependent clinical reactivity, but the known IL-10 induced class switching to IgG4 in humans may be relevant.
In microbicides it is preferable to avoid first line anti-HIV agents that are used to treat HIV-infected people, or agents with the potential to induce class- or cross-resistance to them [9], [10].
Active Ig gene diversification was monitored by ELISA of the RPMI media for the presence of IgG, a result of LPS induced class switch recombination, as described [40], [43] and by ChIPs to show AID association with the 17.2.25 locus (Figure 4B).
These cytokines induce class switching and production of allergen-specific IgE.
In this function, LPS can replace a CD40-derived signal and induce class switch recombination [ 25, 26].
IL-4 activates B cells and induces class switching to the IgG1 isotype (a Th2 type immune response), while IFN-γ induces IgG2a responses (Th1 type) [ 48, 49].
