The signal transduction of most target induced strand displacement-based assays relies on the conformational changes of aptamers, significantly limiting the general applications of these sensors.
Both fluoroquinolones were comparable in their ability to photo-induce DNA strand breaks or oxidative damage to purines and pyrimidines in supercoiled plasmid DNA, but agarose gel electrophoresis showed that BAYy3118 photoproducts could tightly interact with supercoiled plasmid DNA while lomefloxacin ones only induced strand breaks.
Electrochemical aptamer (EA) sensors based on aptamer-cDNA duplex probes (cDNA: complementary DNA) and target induced strand displacement (TISD) recognition are sensitive, selective and capable of detecting a wide variety of target analytes.
Upon MG and CAP adding, the targets could induce strand release due to the highly affinity of analytes toward aptamers.
By binding a ferrous iron complex onto a particular section of a nucleic acid sequence it is possible to induce strand scissions at specific locations [28 30].
This possibility of inducing strand separation due to formation of a G4 DNA could be of functional relevance in processes other than transcription.
Etoposide is a cancer drug that induces strand breaks in cellular DNA by inhibiting topoisomerase II (topoII) religation of cleaved DNA molecules.
Many types of abiotic stress can cause DNA damage, either directly by inducing strand breaks like irradiation or via elevating the level of reactive oxygen species.
H2O2 produces reactive hydroxyl radicals which can induce strand breaks associated with DNA damage.
These alkylating species bind to DNA and induce strand breakage and cross-linking, killing actively replicating cells [ 6].
It is known that EtBr/UV light combination damages DNA by inducing strand breakage and oxidation [ 15, 16].
