RecA induces slippage of paired strands in poly dA)–poly dT) duplex using the energy of ATP hydrolysis.
We show that four and six hours of treatment with actinomycin D or cycloheximide is sufficient to override the mitotic delay caused by nocodazole in NIH3T3 and HEK293 cells, respectively, and induce slippage.
A particularly interesting example of the latter is provided by stretches of small sequence repeats (SSR) that can induce slippage in the processes of replication, transcription, and even translation, and are accordingly sources of localized hyper-mutability (Moxon et al,[17]).
Experimental evidence indicates that expansins can induce slippage of cellulose microfibrils in the cell wall which becomes loosened [ 38].
This phenomenon is particularly evident intraoperatively and is sometimes responsible for difficulties encountered during endodrainage of subretinal fluid from peripheral retinal breaks (i.e., it induces slippage), making it difficult to completely remove the water from the eye [ 74].
There was also no obvious sign of pseudogenization in ruvA, as it does not contain any inactivating mutation (i.e., frameshift or premature stop codon) or homopolymeric tract of poly(A) (>9 bp) which could induce slippage of RNA polymerase (Tamas et al. 2008).
In this paper, we investigated the influences of faults at different stages of evolution on reservoir management and induced fault slippage.
In particular, cracking in this critical region accompanies fixed-end rotation ( theta_{fe} ) in a RC beam, which has been induced from slippage of the main reinforcing steel (( delta ) in Fig. 4) and cannot be simulated by any mechanical model (see Fig. 4).
We previously observed that Fcp1 overexpression in HeLa cells induced faster slippage through SAC activated by the therapeutic AMCD taxol.
For a wide variety of bacteria, this hypermutability is induced by slippage of simple sequence repeats, resulting in insertions and deletions (Kearns et al. 2004; Martin et al. 2005; Moxon et al. 2006).
