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This secondary cytokine wave is produced by cells not infected by virus, but activated by cytokines and cell-cell interactions, which have been induced previously by the initial infection.
Direct block of dendritic calcium channels by local application of the calcium channel blocker Cadmium/Nickel accounted for the majority of the decrease in firing induced previously by GABAB receptor activation.
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However, because these results demonstrate that the amplitude of the stimulus is capable of inducing previously described zone C/E neurons, this experiment does not resolve how the stimulus induces grooming behavior versus wind-induced suppression of locomotion.
GPI-induced arthritis was induced as previously described, with modifications [ 21].
Antigen-induced arthritis (AIA) was induced as previously described and disease severity determined by histological assessment of knee-joint sections.
Furthermore, this work confirms many of the previously in-silico predicted E. coli ncRNAs, and two UV induced peptides previously published as small inner membrane peptides [39].
HAE cells were expanded and differentiation was induced as previously described [40] the generation of cell barrier models for transcytotic assay were carried out as previously described [11].
RT-PCR analysis of stress-inducible genes demonstrated that cellular responses to nsPEFs are distinct from those induced by previously known forms of cellular stress.
Bronchoalveolitis was induced as previously described [5].
Chronic non-bacterial prostatitis were induced as previously described.
Myotube differentiation was induced as previously reported [42].
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