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Histogram shows the global distribution of Hairy peaks around TSS identified from ChIP-seq of induced Flag tagged Hairy protein (right panel) and by previous ChIP chip detection of endogenous Hairy binding (left panel) (MacArthur et al., 2009 ).
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Figure 6A shows a co-immunopreciptiation in which induced Flag-TDP-12xQ/N and Flag-TDP-12xQ/N ▵NRM1/2, ▵N proteins were immunoprecipitated using an anti-Flag antibody as previously described.
MeOH was preferable to 50% TFE, reconstitution in which reduced both p7 functionality and compound sensitivity in subsequent dye release assays (data not shown), as well as to 1,2-diheptanoyl sn-glycero-3-phosphocholine (DHPC) which induced FLAG-p7 oligomerization in our hands (Fig. S1b,d).
Induction of Cre-mediated recombination by TM to label the GFRα1+ and NGN3+ cells with GFP or to induce FLAG-RARγ expression was performed as described previously (Hara et al., 2014; Nakagawa et al., 2010).
To see whether TNF could activate caspase 8 when RIPK3 levels were elevated, we treated WT and Ripk1−/− MEFs with 4HT to induce FLAG-RIPK3, added TNF, and after 4 h lysed the cells and analyzed them by western blot.
This change is similar to that induced by FLAG-TRAK1 overexpression (Fig. 2D).
Subsequently, second-day pupae were heatshocked to induce APPL-FLAG, and the cortical regions of their optic lobes were examined by FLAG immunostaining at 3 hours after induction.
Unless otherwise indicated, HeLa Tet-Off/TTP-Flag cells were grown in the absence of Dox for 48 hr to induce TTP-Flag expression.
However, several of the thirteen ZIFL genes recently described in rice are induced in the flag leaves during senescence [ 15].
We observed the expression of spliced XBP-1 mRNA was strongly induced in ESCs-FLAG-AR, in comparison with ESCs-FLAG.
However, Lap2β-FLAG induced ER complexes which were morphologically similar to those induced by Lap2β-EGFP (Fig. 2C).
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