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The pattern of Wolbachia tissue distribution, infection densities, induced fitness effects, and reproductive manipulations vary greatly within the arthropods and are the result of host and bacterial genotype interactions [ 19- 22].
The rate of decrease depends on the strength of induced fitness costs.
If 4-locus homozygotes are introduced at a higher continuous release rate of 5% per generation, the frequency of individuals carrying ≥1 copy can rapidly increase to very high frequency, reaching ≥96% in 40 generations even in the case of large induced fitness costs of up to 10% per insert.
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This weakness will be exacerbated if the transgenes induce fitness costs.
If mosquitoes are released that are homozygous at 4 loci, a single mass release of 30% can result in over 94% of the mosquitoes in the population possessing at least one copy within 8 generations post-release if the insert does not induce fitness costs.
Antagonistic interactions induce fitness costs on both species: successful interactions come at a cost for species X while unsuccessful interactions come at a cost for species Y.
A recent study demonstrated that deletion of several ORFs can result in compensatory mutations, as would be expected if the initial deletion induced a fitness defect (Teng et al. 2013).
It is now appreciated that, when a population experiences a reduction in size, any valid evaluation of its endangered status requires taking into account the inbreeding depression induced for fitness (Hedrick and Kalinowski 2000), but also that this implies considering the concurrent effects of genetic purging.
In our model for IS-induced fitness changes, we assume that excision or shift events reverse the fitness effect that the IS originally induced.
Functional constraints can induce a fitness cost associated to an antigenic escape mutation.
However, if inserts induce any fitness cost, selection will eliminate the transgene from the population over time.
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