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Doxycycline-induced cells were washed 3 times in PBS followed by 3 cycles of binding buffer for 1 min. In the next step cells were incubated with the AnnexinVC3.18 conjugate (AnnexinVC3.18 binding buffer, 1 100) for 10 min at room temperature, washed 3 times in binding buffer and then subjected to fluorescence microscopy.
Non-induced and induced algal cells were washed with ice-cold water three times.
The non-induced and induced algal cells were washed with sterile distilled water and re-suspended in 8 mg DCW per mL in 50 mL minimal medium supplemented with 5 mM KNO3 and 40 mM D-xylose.
As opposed to XR, XDH could catalyze the oxidation of xylitol to D-xylulose only in the presence of the cofactor NADP+.> -wrap-foot> aThe induced algal cells were washed with sterile distilled water and re-suspended in 8 mg DCW per mL in 50 mL minimal medium supplemented with 5 mM KNO3 and 40 mM D-xylose under light for 24 hours.
Both the un-induced and induced cells were harvested at the exponential phase, cells were washed twice and resuspended in 0.85% sterile saline before being inoculating at ~ 1×109 CFUs/g dw.
The cells were washed with PBS, fixed, and washed again.
The harvested cells were washed and lyophilized.
And cells were washed to remove media.
After the incubation period the cells were washed off and cell retention was calculated.
Cells were washed three times with PBS (5 min for each wash).
Lymphoid cells were washed.
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