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Supervisor support has been found to be a crucial buffer to the negative effects of stress induced by shift work.
(H ) Temperature-sensitive vesicular stomatitis virus G glycoprotein (VSVG-mcherry) traffic to the Golgi induced by shift from restrictive to permissive temperature for 15 min in KDEL-R expressing cells stained for GalNac-T1.
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Differentiation was induced by shifting sub-confluent cultures to DMEM supplemented with 2% horse serum (differentiation medium; DM).
Similar to previous studies of the T. denticola heat shock response [8], [9], heat stress was induced by shifting the incubation temperature from 37°C to 42°C for 1 hour.
Misfolding and degradation can be induced by shifting cells to nonpermissive temperature without inducing a full stress response.
Transgene expression was induced by shifting the growth temperature of the algal culture from 23 to 40°C for 1 h.
Transgene expression was induced by shifting the temperature of the culture from 23 to 36°C for 1 h.
DOI: http://dx.doi.org/10.7554/eLife.00459.008 10.7554/eLiFigure59.009 Figure 1 figure supplement 6. Identification of integral PM proteins induced by shifting cells to 40°C.
Expression of the Kip2 construct was induced by shifting cells from YP-raffinose media to YP-galactose and culturing for a further 16 hr at 30°C.
Myogenic differentiation was induced by shifting the medium to DMEM (GIBCO), containing 2% v/v horse serum (GIBCO) and 1% v/v Penicillin Streptomycin (DM) for 3 days.
Either sporidial cells were directly measured or filamentous growth was induced by shifting to NM (1% glc) and subsequent incubation at 28°C for 6 9 hr.
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