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The knockout of Cacna1g, encoding a T-type Ca2+ channel subunit, confers resistance to chemically and genetically induced absence seizures (24), consistent with the observation that dominant mutations in human CACNA1G can lead to absence epilepsy (25).
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These observations are consistent with the finding that extrasynaptic GABAA receptor gain-of-function in the thalamus is sufficient to induce absence seizures (Cope et al., 2009), and that administration of THIP can initiate spike-and-wave discharges (Fariello & Golden, 1987; Cope et al., 2009).
Differences in passive behaviour induced by absence of desmin were predicted by using a linear model and a non-linear one.
First, the uptake rates into the Xcc wild-type strain were compared after an overnight preculture in the presence (induced) or absence (uninduced) of sucrose.
The decrease in osteoclast number observed in Lmna−/− mice (∼20%) was significantly lower than the decrease induced by absence of lamin A/C on osteoblast number (∼47%, P<0.001) (Figure 2C).
These high levels of MAN-1 expression could be explained by either potentially enhanced transcription or increased protein stability of MAN-1 induced by absence of lamin A/C.
Among these, the V243A mutation induced complete absence of protease activity in vitro, while the V187I mutation induced a marked decrease in its activity.
A fraction of mutant ES cells showed increased γH2AX staining suggesting that chromosomal breaks were induced in absence of OMCG1 (Fig. 6F).
Because the shRNA is not induced in absence of dox, we deemed the –dox condition a better control than the parental cell line.
55 The effect of PDK1 inhibition using short hairpin ribonucleic acid or chemical inhibitors when apoptosis is induced by absence of anchorage, involves antiapoptotic signaling rather than mitogenic signaling.
A striking upregulation of Egr-1 transcription (P < 0.01) was induced in absence of glucose, which was significantly higher than that obtained after 2 hours of 50 mM conditioning in wt cMSCs).
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