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Using the KEA kinase targets analysis, we found that both EGF and IFNγ induce targets of MAPK-1, -3 and −14, as well as of the receptor kinases EGFR, INSR, JAK-1 and −2.
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The other tool, the CRISPR/Cas9 system, can also induce targeted gene disruption by a mechanism distinct from the targeted nucleases described above.
Then lactose was added to a final concentration of 2% (w/v) to induce target protein production.
Nevertheless, the details of exactly how granzyme and perforin cooperate to induce target-cell death remain controversial.
The AOs are evaluated for their ability to induce targeted exon skipping after transfection into cultured myoblasts.
DNA cleavage enzymes can be used to induce targeted mutagenesis of specific genes, including those of exogenous viruses.
Site-specific cleavage of target DNA may induce targeted mutation as a result of error-prone repair by nonhomologous end joining or through homology-directed repair.
Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites.
Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome modifications with high efficiency in cell types including Drosophila, C. elegans, plants, and humans.
Here we test the utility of another type of chimeric nuclease based on bacterial TAL effector proteins in order to induce targeted mutations in silkworm DNA.
Based on these, we designed a cell-based therapy for directing peripheral blood mononuclear cells to induce target cancer cell apoptosis.
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