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The inability of soluble FtsY to induce targeting is puzzling in light of biochemical data which have localized more than 60% of FtsY to the cytosol in E. coli [ 15].
The MTT assay showed that SKOV3-gal cells were killed when culturing with both PBMC and serum, implying that anti-Gal antibody in ovarian cancer patients' serum can bind with SKOV3-gal cells and induce targeting of tumor cells to APCs.
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spear phishing, n.: the fraudulent practice of sending emails ostensibly from a known or trusted sender in order to induce targeted individuals to reveal confidential information.
The AOs are evaluated for their ability to induce targeted exon skipping after transfection into cultured myoblasts.
DNA cleavage enzymes can be used to induce targeted mutagenesis of specific genes, including those of exogenous viruses.
Site-specific cleavage of target DNA may induce targeted mutation as a result of error-prone repair by nonhomologous end joining or through homology-directed repair.
Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites.
Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome modifications with high efficiency in cell types including Drosophila, C. elegans, plants, and humans.
Here we test the utility of another type of chimeric nuclease based on bacterial TAL effector proteins in order to induce targeted mutations in silkworm DNA.
Further, LLO-containing liposomes also recruited LC3-GFP, indicating that LLO was sufficient to induce targeted autophagy in the absence of infection.
These studies suggested that TALEN can serve as a more powerful tool to induce targeted GSV.
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