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The other tool, the CRISPR/Cas9 system, can also induce targeted gene disruption by a mechanism distinct from the targeted nucleases described above.
HEases have been designed to target disease-associated genes, such as XPC [ 7] and RAG1 [ 8], to induce targeted integration in cell lines [ 9, 10], and for targeted mutagenesis in crop [ 11].
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The AOs are evaluated for their ability to induce targeted exon skipping after transfection into cultured myoblasts.
DNA cleavage enzymes can be used to induce targeted mutagenesis of specific genes, including those of exogenous viruses.
Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome modifications with high efficiency in cell types including Drosophila, C. elegans, plants, and humans.
Similar(18)
Protein stability can be efficiently controlled by conditional degrons, which induce target protein degradation at restrictive conditions.
Each of these ZFN pairs induces targeted insertions and deletions with high efficiency at its endogenous gene target in somatic zebrafish cells.
All the designed sgRNAs induced targeted somatic mutations with high efficiency (44.8 100%; average, 86.8%).
Auxin induces targeted ubiquitination/degradation of specific AUX/IAA proteins [ 37] and frees ARFs from repression by AUX/IAA proteins.
RNAa is a small RNA-guided and Argonaute-dependent gene regulation phenomenon in which promoter-targeted short double-stranded RNAs (dsRNAs) induce target gene expression at the transcriptional level.
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