Then lactose was added to a final concentration of 2% (w/v) to induce target protein production.
Based on these, we designed a cell-based therapy for directing peripheral blood mononuclear cells to induce target cancer cell apoptosis.
As the major downstream effectors of the Hippo pathway, unphosphorylated YAP/TAZ translocate to the nucleus and induce target gene transcription by interacting with the transcription factors TEADs (TEAD1 4).
Such small-molecular, amide-type SNIPERs that induce target protein-selective degradation without affecting IAPs should be effective tools to study the biological roles of target proteins in living cells.
These results suggested that only a sufficient concentration of dsRNA can induce target gene knockdown.
4-NP has been shown to induce target genes in a number of arrays and in other systems.
A few studies have shown that antisense and peptide aptamers targeting HPV E6/E7 can induce target cell apoptosis [23], [24], [25].
Regardless, it is likely that more than the type I repeat region of TSP-1 is necessary for LAP to induce target cell activation.
Circadian rhythm molecule ARNTL/Bmal1 is the obligatory partner of NPAS2 to induce target genes through a cis-acting MOP3/MOP4 responsive element (M34RE; CACGTGACC) [44].
We examined the guide's capacity to induce target cleavage by RNase IIIs from bacteria (RNase III), fission yeast (Pac 1) and human (Dicer) (Figures 3C and D).
Of note, normally low Cre concentrations suffice to induce target DNA rearrangement with subsequent signal amplification being relayed via reporter gene transcriptional activity.
