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Both recipient and pollen donor identity affected onset of stigma receptivity within populations, confirming previous results that some pollen donors can induce stigma receptivity.
In this study, the two investigated regions of C. heterophylla differed in flower size and pollen tube growth rate, a pollen trait probably unrelated to the ability to induce stigma receptivity [20].
In our case it could be hypothesised that C. heterophylla in the two regions are too diverged in traits related to mating system, so that pollen fail to induce stigma receptivity on recipients of other regions.
A recent phylogenetic analysis of C. heterophylla further indicates that these two regions represent separated clades (unpubl. data, B. Baldwin et al).. Population relatedness indeed mattered when interpreting the outcome of the crosses because the ability to induce stigma receptivity in a foreign population was different depending on how closely related the foreign population was.
Because the results of our crosses indicated that the ability to induce stigma receptivity in a foreign population may be different when the foreign population was more distant (see results), we also examined if recipient costs of early fertilisation differed between crosses within or between regions.
Even though donors that produce early-germinating pollen will also be affected by this cost, the ability to induce stigma receptivity could still be selected for if this trait results in a higher fertilisation success (e.g. by securing paternity) [cf. 10].
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Although many wanted to make more effort to market items directly to WIC customers to facilitate the shopping experience, they were concerned about inducing stigma by making it more obvious which customers were WIC participants.
Pollen donors were less capable of inducing stigma receptivity on recipients of the other region.
For example, it is possible that the benefit of inducing stigma receptivity may be reduced if this manipulation also facilitates the germination of other pollen grains [cf. 17].
On the other hand, donors were less capable of inducing stigma receptivity in crosses between different regions than in crosses between populations belonging to the same region.
In crosses between regions, timing of stigma receptivity appeared later compared to crosses within regions, i.e. donors were less capable of inducing stigma receptivity on recipients originating from another region (Figure 1).
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