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Wild-type Smad4, but not DNA-binding-deficient mutant Smad4-D4 [20], wableble to induce promoter activity when cotransfected with Smad1 (Fig. 2B).
The 5' deletion analysis of the MAG promoter revealed that the 20-bp sequence extending from nucleotide −162 to −143 could induce promoter activity at levels similar to that induced by the longest reporter construct.
To unravel the mechanism by which IgE engagement of FcεRI on cytokine pre-sensitized B/TSM leads to enhanced CC and CXC chemokine release, we then tested whether IgE alone can induce promoter activity of CCL11, CXCL8, CXCL10 and CCL5 in B/TSM cells.
We also found that NRPs induce promoter activation and expression of GmNAC6 genes.
However, treatment with the RAR agonist all-trans RA failed to induce promoter activity, indicating the specificity of this promoter construct.
Next, we tested whether mutationally activated β-catenin was able to induce promoter activity from pTopFlash in TCC and NUEC in cotransfection experiments.
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Moreover, long-term, high transactivator levels in neurons can often overcome integration-induced Ptet gene silencing, possibly by inducing promoter accessibility.
However, it is noticed that heat shock induced promoter activities of the Drosophila 194 bp and the 1.1 kb hsp70 promoter were different at 37°C in A. aegypti.
Smad4 alone induced promoter activation.
Here, H. pylori-induced promoter methylation is observed not only in tumor suppressor genes but also in microRNAs (miRNAs).
In this study, we found that androgen is capable of inducing promoter activity of GNMT through the AR signalling pathway.
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