Over the last decade several new developments in FEBID and focused electron beam induced processing (FEBIP) have led to a growing number of scientific contributions in solid state physics and materials science based on FEBID-specific materials and particular shapes and arrangements of the employed nanostructures.
The NLRP3-inflammasome is a signaling complex that activates procaspase-1 and induces processing of caspase-1 dependent inflammatory cytokines (particularly IL-1β and IL-18).
Consistent with previous reports [23], [24], CD40 ligation induced processing of NF-κB2 as well as upregulation of p100 and RelB, indicating the activation of both canonical and alternative pathways, whereas LPS treatment induced only the canonical pathway.
Correspondingly, western blot analysis of control B cell lysates demonstrated that BAFF induced processing of p100 to p52 but did not upregulate p100 or RelB in control B cells, suggesting specific activation of the alternative NF-κB pathway (Fig. 4b).
Subsequently, NIK induces processing of p100 by IKKα homodimers, resulting in partial degradation into p52.
To determine whether chronic SCI induces processing of caspase-1 and the pro-inflammatory cytokines IL-1β and IL-18, we analyzed VAT and pancreatic lysate, comparing chronic SCI, naïve and sham-operated control.
TRAIL induced processing of pro-caspase-3 to its p20 fragment, but not to the fully processed p17 fragment, detectable in the TRAIL-sensitive Colo205, colon carcinoma cells or in hFb pre-treated with 10 μg/ml CHX for 15 h.
Upon miniaturisation, however, the influence of the affected region becomes more important and may induce a processing induced size effect, which is far from negligible.
FCCP-induced depolarization was previously shown to induce rapid processing of long Opa1 to short Opa1 forms that do not mediate fusion per se (Guillery et al, 2008).
When not utilized carefully, all these operations can induce tremendous processing overheads in practical settings.
