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These peptides generally exhibit a broad range of bactericidal activity which requires a short contact time to induce killing.
The low concentrations of ATTM and 2ME2 employed in the RAD54B SLD1 SL paradigm are purposefully selected to induce killing only within the RAD54B-deficient cells and are therefore not expected to produce side effects within normal surrounding cells.
Strikingly, in vitro expression of HlgAB, LukED, and HlgCB was highly variable among bovine mastitis isolates and some strains did not produce functional levels of these toxins, i.e. levels sufficient to induce killing of HEK293T cells bearing their cognate receptor.
Properties such as decreased binding to anti-SEA as well as higher selectivity to induce killing of tumour cells compared to MHC class II expressing cells, were sequentially improved.
The non-vesicular fractions did not induce killing of the smooth muscle cells (Figure 3C).
In contrast, celecoxib alone did not induce killing of A431 or MDA-MB-231 cells.
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CD40 induces killing of T. gondii independently of IFN-γ, STAT1, NOS2, Irgm1, Irgm3 and Irga6 [8], [33] [51], [51].
Neutralization titers were defined as the reciprocal serum dilution (before the addition of cells) at which 50% of cells are protected from virus induced killing.
IFN-γ induces killing of type II strains of the parasite through Atg5-dependent recruitment of Irga6 to the membrane of the parasitophorous vacuole [13].
Notably, neither did rapamycin restrict replication of M. tuberculosis or BCG nor did WM abrogate IFN-γ induced killing (Fig. 1 D and data not shown).
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