CSLCs are metabolically distinct from differentiated cells, and any aberration from the intrinsic metabolic state can induce differentiation of CSLCs.
Dihydrotestosterone has been shown to further induce differentiation of prostate sphere cells60.
The growth factors were removed and T3 (60 nM) was added to induce differentiation.
ATRA was known to induce differentiation of leukemia cells and squamous cancer cells38,39.
Of note, HoxA9 knockdown has been shown to induce differentiation, cell cycle arrest and apoptosis of AML cells.
The addition of insulin, triiodothyronine and ascorbic acid (ITA) to the culture was sufficient to induce differentiation.
Yan, Y. et al. All-trans retinoic acids induce differentiation and sensitize a radioresistant breast cancer cells to chemotherapy.
To induce differentiation, 80 90% confluent cells were cultured in differentiation media (DMEM with 2% horse serum).
Therefore, we thought Foxc1 could be another candidate to induce differentiation of the primitive OE into salivary gland rudiment.
Ochiai, H. et al. BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm.
IMR-90 fibroblasts were treated with doxycycline (DOX) to induce MYOD1 expression and then switched to DM to induce differentiation.
