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Interestingly, SIRT1 is required to induce differentiating NSCs to adopt an astrocytic fate under oxidative stress.
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DNA adducts induced in differentiating or stem cell spermatogonia, however, did not persist to spermatozoa.
How is D2 induced in differentiating myoblasts?
Confluent preadipocytes were induced to differentiate using a standard differentiation medium (DM2-L1, Zen-Bio Inc, Research Triangle Park, NC, USA).
In NSPCs induced to differentiate, overexpressing miR-106b~25 enhances differentiation toward the neuronal lineage.
We originally identified Fam20a as a differentially expressed mRNA in EML cells induced to differentiate along the myeloid lineage.
NSC cultures were induced to differentiate for 3 weeks in NSC differentiation media.
HUES-9 NSC cultures were induced to differentiate for 3 weeks in neuron differentiation medium.
For example, Muse-AT cells demonstrated formation of lipid droplets when induced to differentiate into adipocytes (Figure 4A), as well as characteristic smooth muscle striations when induced to differentiate into myocytes (Figure 4B) [26].
MSCs on PCL nanofibers were also induced to differentiate along adipogenic, chondrogenic and osteogenic lineages in specific differentiation media [19].
The putative CSCs were induced to differentiate into different lineages using adipogenic, chondrogenic, and osteogenic differentiation media (PromoCell, Heidelberg Germany).
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