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Comparison of cases of RA versus control individuals yielded a χ2 value of 14.28 P=.00022), with a genotype odds ratio of 2.24 (95% confidence interval [95%CI] 1.44 3.49).
The 91 DH individuals yielded a total of 212.3 million PE reads, ranging from 0.57 to 9.79 million reads in different DHs with an average of 2.33 million reads per DH line (Additional file 1: Figure S2).
Sequencing of ITS1 in 282 individuals yielded a fragment of ca. 1,100 bp, 155 bp of it belonging to the highly conserved 18 S rDNA region, and the remaining to the ITS1 region.
The anamnestic data of all individuals yielded a more frequent presentation of subjective symptoms and dysfunctions from the sample of RA patients (Group RA) than from the control group (group N) (Table 1).
For the introgression of xol-1 tm3055) xol-1 tm3055e above), we used PCR genotyping with the forwA6140rimerseeOL2L: 5′-GGGaboveTAGGAGCGAAA-3′, XOL4L: 5′-ATGATTGATGATTTACCGAAGC-3′ and the reverse primer XOL2R: 5′-GGGATTGAGCACCAAACTT-3′. xol-1 (we/wt) individused yielded a single 471 bPCRand, xol-1 (tm3055/tm3055) one 375 bp band, and the heterozygenotyping.
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Only the cytb PCR for Mexican Altiplano individuals yielded an additional, unexpected band.
Detection of GLP-1 after 1 to 4 freeze/thaw cycles in probes from 3 different individuals yielded an intra-assay variability of 9,23% which is comparable to the general intra-assay variability.
Each individual yielded a unique 16S rDNA sequence in 1 2 teeth per individual.
In this study, we observed that not all dental pulp specimens collected from a single individual yielded a positive PCR product.
Illumina sequencing of one I. elegans individual yielded a total of 110 Gb of mRNA sequence equivalents consisting of 428,744,100 paired-ends 100 bp reads (155,232,504 reads from the head, 159,734,116 from the thorax and 113,777,480 from the abdomen, respectively).
Ponderosae individuals yielded 45 total alleles, with a mean of 7.5 (2.3 SD) alleles per locus (Table 3).
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