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Finally, 4419 markers sites and 177 F2 individuals were used.
Because the BEAGLE algorithm requires phased data, only diploid individuals were used.
Eight individuals were used to examine amplifications and polymorphisms for all 50 loci.
The PCR-positive individuals were used for further analyses (Figure 1B and Table 2).
These individuals were used to identify what CoS meets the user requirement.
For each concentration, 20 daphnids divided into 4 groups of 5 individuals were used.
Three individuals were used for whole fish, muscle, and liver (n = 9 fish), respectively.
In optimization by genetic algorithm, chromosomes with four genes and the primary population with 10 individuals were used.
For chromosome X, only female individuals were used; otherwise, all unrelated individuals were used.
A total of 144 individuals were used as subjects.
Metaphases of different individuals were used for FISH analysis.
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