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PBMC from patients and control individuals were stimulated with SEA, PPD, ESAT-6/CFP-10 and BCG or left un-stimulated for 18 or 72 hours and then analysis of cytokine secreting cells was performed.
Only CD4+ T cells from tolerant individuals were stimulated by BB, and levels of Th2 cytokines were 80% lower.
PBMCs from tolerant individuals were stimulated to proliferate only with BB, and they secreted significantly lower levels of Th2 cytokines.
To characterize virus-specific cellular T cell cytokine induction kinetics, one million PBMCs from 4 HIV positive individuals were stimulated with the predefined HIV CD8+ peptide epitope pool for 12 hours; and ex vivo cultured cells were harvested at hourly intervals for the first six hours and then once every two hours up to 12 hours.
This correlation arose because individuals were stimulated to leave the vegetated area because others were located near the predator.
Monocyte-derived dendritic cells from five allergic individuals were stimulated with Pam3CSK4, FSL-1, LPS, MPL-A, or flagellin.
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Demographic and clinical characteristics of the 24 patients with active tuberculosis and 28 patients with successfully treated tuberculosis are summarized in table 1. Whole blood from all individuals was stimulated with PPD, ESAT-6 and CFP-10, and antigen-specific CD4 T cells co-expressing CD69, IFN-γ and/or IL-2 were quantified using flow-cytometry.
While at work, individuals are stimulated by physical and mental activities and social contacts.
PBMC from one individual were stimulated with iZEBOV on day 0 and again on day 6.
APCs (MΦs and moDCs) were taken from these healthy and L. donovani infected individuals and were stimulated with recombinant protein (KMP-11) prepared to look at the immunological response of the cells.
To assess the immunogenicity of CD4+ T cell epitopes in vivo, splenocytes from mice immunized with individual peptides were stimulated with the corresponding peptides.
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