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Some individuals were singly infected with either type 1 or type 2 EBV, whereas some were co-infected with both types of EBV.
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Since the cohort included healthy individuals that were singly sampled (cross-sectional), phylogenetic analyses incorporating SA MLST data from these individuals were also performed (Additional file 3: Figure S1, Supplemental Information) and the analyses reveal that all SA carrier strains within the cohort are highly similar to strains of clinical origin.
For aggression assay, individual males were singly housed for 2 3 weeks.
Before the experimental trials, individuals were housed singly in separate cages.
Individuals were placed singly in 200 μl of fresh medium in 500 μl plastic tubes, with fresh medium (100 μl) added once, on day 10 after isolation.
Individuals were kept singly in wire-netting rectangular containers (20 × 10 × 15 cm; L × W × H) placed in a tank (90 × 40 × 40 cm; L × W × H) with a depth of 10 cm of fresh water at room temperature.
They were singly housed in individual cages on a 14-h light/10-h dark cycle (lights on at 7 00 am) and allowed food and water ad libitum.
All mice were singly housed to facilitate individual dietary intake assessment, and received water AL.
Experimental animals were singly housed for procedures requiring individual measurements of food intake or energy expenditure.
After surgery, animals were singly housed for 48 h.
Mice were singly housed and handled for 2 days prior to NE exposure.
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