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All reads corresponding to each of the individuals were separately aligned using MUSCLE [22] generating one alignment per individual.
Leaves (used for leaf area measurements), residual aboveground and root material of these individuals were separately chopped, finely ground with a ball mill and C and N concentrations were determined using an elemental analyser (Vario EL III Element Analyzer, Elementar, Hanau, Germany).
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Variation between individuals was separately estimated for three variables: how passengers evaluate the capital costs of owning a car; how passengers are willing to pay for either the right to drive themselves or the right not to drive; and how many passengers are travelling together.
Each time-point (6, 12, 24, 48 h) and sham-operated (sh) group consisted three animals per group; RNAs from each individual were separately labelled and analyzed by microarray hybridization, for a total of 15 microarray hybridizations.
Individual models were separately estimated and contrasts were defined in order to pick out the main effects of each experimental condition.
Focus groups, participant observations, audit documents and individual interviews were separately analysed.
In turn, individual coverslips were separately transferred to OptiMEM®+2% FBS and 30 ng/ml VEGF was added.
The complete data set and individual classes were separately analyzed to extract their best features, which were incorporated into a composite model for the complete 80S-Sec61 complex.
Isolated chondrocytes from individual specimens were separately cultured with DMEM/F-12 plus 10% FBS at 37°C under a humidified 5% CO2 atmosphere until reaching confluence.
Individual cell populations were separately evaluated by gating based on forward and side scatter and/or expression of surface markers.
Both young and adult (≥ 35 years) individuals were evaluated separately.
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