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Samples from Armenian and from Karabakhian individuals were processed at the same time using identical experimental conditions.
Two randomly chosen subsets consisting of 9 individuals of the Swiss population and a subsample comprising 7 Swiss and 6 Rwandese individuals were processed on the Nimblegen 385K aCGH array and the Illumina 1M-Duo BeadChip, respectively.
Animal tissue or entire individuals were processed for histology; fixed in buffered formalin (10%), processed using standard technique, embedded in paraffin, sectioned and stained with haemotoxylin and eosin (H&E) or Giemsa (Veterinary Histopathology Laboratory, University of Sydney).
In addition, the fact that HWE deviations were absent in control groups and found in opposite directions in the Armenian and Karabakhian populations of patients also strongly argues against genotyping errors, all the more since all samples (from Armenian and Karabakhian individuals) were processed at the same time and following identical experimental procedures.
The sera of six breast cancer patients with metastasizing disease and six control individuals were processed with MBD loaded beads.
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Raw acceleration data files from each individual were processed using a bespoke computer programme (MAHUffe, http://www.mrc-epid.cam.ac.uk/Research/Programmes/Programme_5/InDepth/Programme%205_Downloads.html).html
All samples for a given individual were processed on the same slide.
The raw pyrosequencing data obtained from a cDNA library of a single individual were processed using CLC Genomics Workbench CLC Bioo, Denmark).
1 Two aliquots of a DNA sample from this individual were processed in parallel and pooled for sequencing. 2 A DNA sample from this individual was sonicated, then divided into two aliquots for subsequent library construction steps.
To avoid sample mix up, each individual was processed separately.
Since the year 2000, 443 individual cases were processed.
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