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The organs from eight individuals were pooled for each biological replication.
In order to minimize genetic variability melanocytes from 4 5 individuals were pooled for each culture.
For each species, detection data from all individuals were pooled before analyses.
DNA samples from leukocytes of 400 healthy individuals were pooled together as a group with an aggregated concentration of 200 ng/µl.
Whole organism samples were from 30 larvae of 2nd and 3rd instars, whereas 10 individuals were pooled from each stage of 4th instars, pupae, and adults.
The tissues of 30 individuals were pooled into microtubes containing 60 µl sterile H2O and stored at −20°C until analysis.
Similar(12)
Hemolymph from four individuals was pooled, and five biological replicates were taken at each sampling point.
For each population, genomic DNA for ten individuals was pooled together equally.
At the end of one generation all offspring produced by the individuals are pooled.
To generate the common reference pool, 10 μg of aRNA from each of the twenty-four 9 mm individuals was pooled.
The main limitation is that individual genotypes and haplotypes cannot be extracted because the DNA of individuals is pooled.
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