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For each species, liver tissues from three individuals were mixed.
Cells from different individuals were mixed in equal proportions, and SCIDs and SHEDs were cultured separately.
RNA extractions of 5 individuals were mixed in each replicate to equal quantities (2 mg) for later microarray analysis.
To test the suitability of the DNA pooling approach, and to ensure that rare alleles would be detected, thus avoiding false negative results, DNA from two individuals were mixed in different proportions raging from 1 0 to 1 10.
On the third generation, L1-staged GFP individuals were mixed with wild type experimental individuals at expected relative frequencies of 50%; see [ 70] for a density calibration curve of this protocol.
For each line equal quantities of DNA from 10 15 individuals were mixed to produce pooled samples, except for one line for which three samples were sequenced individually (see Table 1).
Similar(51)
They establish mutually favorable territories based on the throat visual cues, even when unrelated individuals are mixed.
Fragmented, tagged samples from different individuals are mixed in equal amounts to form a single pool of DNA molecules.
DNA from normal individuals was mixed with the mutated DNA to obtain a final dilution of 10, 5 and 1 %.
Note that in the null model, the individuals are mixed each time step, which means each individual is moved to a random location on the grid.
The de-mixing problem is trivially solved when only two independent components (e.g. from two different individuals) are mixed together, and their copy number, a ji + b ji, is measured using independent experimental approaches in at least two loci.
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