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All individuals were maintained in small groups (less than 20 individuals) on a standard yeast-cornmeal agar food under a 12L:12D cycle at room temperature.
All of the individuals were maintained in plastic containers (60×40×40 cm) at 20°C until their arrival at the laboratory, where they were maintained at 10°C in a climate chamber (a temperature similar to the average one experienced in the field during autumn).
These individuals were maintained in field by Suzano Papel and Cellulose breeding program.
With R set at 1.6, approximately 1000 individuals were maintained in a 1000 × 1000 habitat when K = 10.
We performed intent-to-treat analysis where these individuals were maintained in all outcome measures preserving randomization.
Male and female juvenile individuals were maintained in the laboratory in 10 L tanks of aerated, filtered, sea water at 18 ± 1°C.
Similar(51)
Clonemates were separated so that each individual was maintained in its own chamber in control (45 Pa) or hypercapnic (231 Pa) conditions.
Cortical neurons from individual pups were maintained in Neurobasal medium (Gibco) containing 10% fetal calf serum, at 37°C in humidified air containing 5% CO2.
Individual cultures were maintained in 100 mL of ASTM hard synthetic water at low and high food-ration levels (Chlorella vulgaris, 1 × 10 and 5 × 10 cells/mL, respectively), as described by Barata and Baird (1998).
In our model, with K = 10, about 1000 individuals (500 males) were maintained in the 1000 × 1000 cells habitat.
The individual cell lines were maintained in Opti-MEM media supplemented with 10% FBS and 4 µg/mL puromycin.
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