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The T0 individuals were inoculated with Xoc strain RS105 along with Shengdao806 (WT) at the seedling stage.
A total of 637 F2 individuals were inoculated with M. oryzae isolate 318 2, which was incompatible to both Jefferson and 75-1-127, theobserve the phenotype segregation.
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One group of individuals was inoculated with purified AbSV genomic DNAs through syringe injection, while the negative control group was inoculated with artificial sea water in parallel.
Cultures derived from one individual were inoculated with 0.5 multiples of infection of influenza A/WSN/33 virus, as previously described [ 12].
Individual birds were inoculated at 15 d of age with sporulated oocysts from E. acervulina (30,000), E. maxima (10,000) or E. tenella (2,000).
Three individual colonies were inoculated in SC-trp to allow for retention of the pGBKT7 episomes and for loss of pARS1wt and grown for 20 generations.
Earthworm microcosms were developed based on a combination of published protocols [75], [76] whereby non-sterile soil containing individual animals were inoculated with ∼5×109 bacteria and incubated for 1 week, prior to 22 subsequent weeks marked by repeated passages through "clean" soils (i.e., no B. anthracis).
After selection for 3 days, individual colonies were inoculated in selective medium at 30°C overnight.
Individual wounds were inoculated with 10 bacteria on postoperative day (POD) 3 and redressed with Tegaderm.
Three biological replicates (individual plants) were inoculated in each treatment group.
Individual colonies were inoculated into -ura -trp SC medium with 2% dextrose and grown to mid-log phase.
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