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Another 20 individuals were injected with 1× phosphate-buffered saline as a control.
Fifty individuals were injected at base of the fifth leg with 100 μL GnRH solution at the concentration of 500 ng GnRH/g body weight (BW).
Fifty individuals were injected per treatment for each replicate, and experiments to confirm each phenotype reported here were repeated in triplicate.
At 4.5 months of age, 12 individuals were injected with 100 μl of 106 p.f.u./ml of ATV isolated from the axolotl colony and suspended in cell culture medium.
In summary, individuals were injected intraperitoneally at 0 hr with a phytohemagglutinin solution to stimulate mitosis, injected at 24 hr with 1% colchicine solution, and then killed at 26 hr using MS-222.
Although previous reports have shown a limited induction of Aβ aggregation when brain samples from non-demented individuals were injected to tg2576 [ 22] and APP23 [ 23] mice, no reports have shown before a comparison with the pathological induction produced by an AD brain.
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The severity of the disease can be reduced if the affected individual is injected within two weeks of exposure with immune serum globulin obtained from persons exposed to HAV.
Each individual was injected on a weekly basis.
To minimize potential variation of individual mice, individual mice were injected with all three transducing viruses s.c. at the abdominal area as shown in Figure 4B.
In contrast, the individuals that were injected with Pahsp70 dsRNA were not able to repair heat injury and all died within 3 days (Fig. 2F).
Individual snails were injected intravaginally with a peptide or protein at a biologically relevant concentration.
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