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Before the flight test, focal individuals were gently collected and chilled for 20 s in a petri dish placed in direct contact with ice.
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One flower of an apomictic polyploid individual was gently rubbed on the flowers of a sexual individual during the period of stigma receptivity.
Twenty individual flies were gently introduced into a glass vial height 240 mm tall and 25 mm in diameter.
For in situ recording, individual slices were gently transferred into a recording chamber which was constantly perfused with isoosmotic solution (2.5 ml/min).
Individual mice were gently transferred to a 12.7×7.6×5.1 cm (width × depth × height) closed box with a 1.27 cm square hole on a side wall.
Individual slices were gently transferred to normal artificial cerebrospinal fluid (ACSF) for at least 1h prior to recording.
0.5 ml of each individual gradient solution were gently layered into a 4.5 ml centrifuge tube (Beckman, 344062) from the heaviest to the lightest solution, as previously described [ 40].
Individual intact test males were gently aspirated into the chamber containing a decapitated target and the behavior of the test males was recorded for 45 min. Round chambers of 10 mm diameter and 4 mm height were used for the mating competition assay.
Individual one-day-old flies were gently flipped onto their backs, and the time it took to right themselves to a standing position was clocked by stopwatch.
After uptake, 50 individuals at the 1st larval instar were gently placed on the leaves for feeding.
Recent schools of academic thought have suggested that individuals can be gently coaxed into doing so if they are given the right frameworks.
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