All the individuals were allocated to groups according to their diagnosis: myofascial pain (group A, 676 subjects, 525 females and 151 males; mean age ± SD = 35.2 ± 12.6), migraine (group B, 39 subjects, 29 females and 10 males; mean age ± SD 36.0 ± 10.7), and both myofascial pain and migraine (group C, 66 subjects, 56 females and 10 males, mean age ± SD = 35.6 ± 10.8).
In particular, individuals were allocated to classes or clusters on the basis of highest membership probability.
All individuals were allocated to treatments as young larvae, i.e. ca. 20 days prior to testing temperature stress resistance.
Using the 42 individuals of the F0 group as putative founders, all 20 F1 individuals were allocated to the correct parent pair with a 100% accuracy.
After individuals were allocated to one of the sampling units, variable means were calculated from the raw data for the following variables: LGO, BPX, LBN, HBB, AFR, APA, AOC, CFR, COC, HNP, HNZ, BNZ, BZY, BFW, BFX, HORC (Table 4).
On their eclosion day, butterflies were randomly allocated to different adult treatment groups as outlined below, except for experiment 9, where individuals were allocated to treatments as young larvae already (see below).
The cluster randomized trial, in which groups rather than individuals are allocated to different interventions, is an increasingly popular design.
Individuals are allocated to a class according to a non-parametric marginal probability.
Baucha et al. [20] developed an age-structured MSEIRV compartmental model, whereby individuals are allocated into one of a number of mutually exclusive categories based on their epidemiological status and age.
