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DNA from 1286 of those individuals was successfully amplified and used for genetic analysis.
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While biogeographic isolation in part explains low localised densities of non-endemic species, overall numbers per species indicate that many individuals are successfully arriving at LHI, but that sediment conditions – particularly in the drowned lagoon and to a lesser extent across the relict reef – may be too dynamic to provide suitable habitat for many of these species.
The majority of the sampled individuals were successfully contacted in one of the first two contact attempts.
In this study, all fish (50 individuals) were successfully trained to accept the FT pellets after 11 days of conditioning.
4464 individuals were successfully genotyped on the admixture panel [24], [13], [25].
All individuals were successfully genotyped at call rate >98.1% with genotype call threshold of 0.15.
A total of 18,494 individuals were successfully interviewed giving a pooled response rate of 98%.
All individuals were successfully genotyped for the SNPs rs653765 and rs514049.
All individuals were successfully genotyped at the remaining nine microsatellite markers.
Diploid individuals were successfully genotyped for the 3472 SNPs included in the linkage map using Stacks.
99.1% of the collected samples (3481 individuals) were successfully genotyped at more than 10 loci, and 99.8% of the collected samples (3507 individuals) were successfully genotyped at more than 6 loci.
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